Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 523 |
| PDBID: | 3IYW |
| Chains: | KM_C |
| Organism: | West Nile virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 13.70 |
| Reference: | 10.1073/pnas.1011036107 |
| Antibody | |
| Antibody: | CR4354 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | West Nile virus Polyprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >3IYW_K|Chain D[auth H], F[auth K]|CR4354 Fab fragment X1, heavy chain H|Homo sapiens (9606) QVQLVQSGAEVRKPGASTKVSCKASGYTFTHYYMHWVRQAPGQGLEWMGIINPSGGSTTYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDWGSNYVWGSYPKYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTH |
Light Chain: M
Mutation: NULL
| >3IYW_M|Chain E[auth L], G[auth M]|CR4354 Fab fragment X1, light chain L|Homo sapiens (9606) QSVLTQPSSVSGTPGQRVTISCSGSSSNIGSNTVNWYQQLPGTAPKLLIYGNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLNGPVFGGGTKLTVLGAAAGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: C
Mutation: NULL
| >3IYW_C|Chain A, B, C|Envelope glycoprotein|West Nile virus (11082) FNCLGMSNRDFLEGVSGATWVDLVLEGDSCVTIMSKDKPTIDVKMMNMEAANLAEVRSYCYLATVSDLSTKAACPTMGEAHNDKRADPAFVCRQGVVDRGWGNGCGLFGKGSIDTCAKFACSTKAIGRTILKENIKYEVAIFVHGPTTVESHGNYSTQVGATQAGRFSITPAAPSYTLKLGEYGEVTVDCEPRSGIDTNAYYVMTVGTKTFLVHREWFMDLNLPWSSAGSTVWRNRETLMEFEEPHATKQSVIALGSQEGALHQALAGAIPVEFSSNTVKLTSGHLKCRVKMEKLQLKGTTYGVCSKAFKFLGTPADTGHGTVVLELQYTGTDGPCKVPISSVASLNDLTPVGRLVTVNPFVSVATANAKVLIELEPPFGDSYIVVGRGEQQINHHWHKS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: TYR27 THR28 PHE29 THR30 HIS31 TYR32 TYR33 ASN52 SER54 GLY55 ARG72 THR74 SER75 SER77 TRP100 GLY101 SER102 ASN103 TYR104 LYS111 TYR112 M: TYR50 PRO56 SER57 C: ASN47 GLU49 ALA50 ALA51 ASN52 GLU133 ASN134 ILE135 LYS136 TYR137 GLU138 TYR155 SER156 VAL159 ARG166 PHE167 SER168 THR170 PRO171 ALA172 TYR176 SER276 ASN277 THR278 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
