Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5226 |
| PDBID: | 7UVO |
| Chains: | AB_C |
| Organism: | Homo sapiens, Plasmodium falciparum |
| Method: | XRD |
| Resolution (Å): | 2.09 |
| Reference: | 10.1016/j.immuni.2023.01.013 |
| Antibody | |
| Antibody: | RUPA-38 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum gametocyte surface protein P230 domain 1 (Pfs230D1) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7UVO_A|Chain A|RUPA-38 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGRSLRLSCAVSGFNFEDYAMHWVRQTPGKGLEWVSGISWNSGTLAYGDFVQGRFIISRDNAKNFLYLQMNSLRPEDTALYYCANDRMRWIQVPHYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: B
Mutation: NULL
| >7UVO_B|Chain B|RUPA-38 Fab light chain|Homo sapiens (9606) LSALTQPRSVSGSPGQSVTISCTGTNDDVGEYNYVSWYQQHPGKAPKLMIYDVTKRPSGVPDRFSASKSGNTASLTISGLQAEDEANYYCCSYAGTYHMVFGGGTKLTVLVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECC |
Antigen
Chain: C
Mutation: NULL
| >7UVO_C|Chain C|Gametocyte surface protein P230|Plasmodium falciparum (5833) VGVDELDKIDLSYETTESGDTAVSEDSYDKYASQNTNKEYVCDFTDQLKPTESGPKVKKCEVKVNEPLIKVKIICPLKGSVEKLYDNIEYVPKKSPYVVLTKEETKLKEKLLSKLIYGLLISPTVNEKENNFKEGVIEFTLPPVVHKATVFYFICDNSKTEDDNKKGNRGIVEVYVEPYGGSLKENLYFQGWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TRP52 ASN53 SER54 GLY55 THR56 LEU57 ALA58 TYR59 GLY60 ASP61 GLN64 ARG98 TRP99 B: GLU29 TYR30 TYR32 TYR91 GLY93 THR94 HIS95 C: ASP561 SER563 TYR564 GLU565 THR566 THR567 GLY570 ASP571 THR572 ALA573 LYS629 ASN638 GLU640 VAL642 LYS644 LYS657 ILE705 ASP707 SER709 LYS710 ARG720 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
