Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5215 |
| PDBID: | 7URX |
| Chains: | HL_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1126/sciimmunol.adf1426 |
| Antibody | |
| Antibody: | SJ25C1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human B-lymphocyte antigen CD19 |
| Antigen mutation: | No |
| Durg Target: | P15391 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7URX_H|Chain C[auth H]|SJ25C1 Fab heavy chain|Mus musculus (10090) MGWSCIILFLVATATGVHSEVKLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHHHH |
Light Chain: L
Mutation: NULL
| >7URX_L|Chain B[auth L]|SJ25C1 Fab light chain|Mus musculus (10090) MGWSCIILFLVATATGVHSDIELTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRTRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7URX_C|Chain A[auth C]|B-lymphocyte antigen CD19|Homo sapiens (9606) MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHVSPLAIWLFISNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLY49 THR50 TYR68 TYR72 H: GLY45 TYR46 ALA47 SER49 SER50 TYR51 TRP52 TYR71 GLY73 ASP74 ASP76 THR119 ILE120 SER121 SER122 VAL123 VAL124 PHE126 TYR127 C: LEU96 SER103 LYS105 TRP107 GLU120 LYS155 TYR157 TRP159 ASP162 ARG163 PRO164 GLU165 ILE166 GLU168 THR215 VAL217 HIS218 PRO219 LYS220 GLY221 PRO222 SER224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
