Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5214 |
| PDBID: | 7URV |
| Chains: | D_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.05 |
| Reference: | 10.1126/sciimmunol.adf1426 |
| Antibody | |
| Antibody: | FMC63 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human B-lymphocyte antigen CD19 |
| Antigen mutation: | No |
| Durg Target: | P15391 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7URV_D|Chain B[auth D]|FMC63 single-chain variable fragment|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGGGGSGGGGSGGGGSEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSS |
Antigen
Chain: C
Mutation: NULL
| >7URV_C|Chain A[auth C]|B-lymphocyte antigen CD19|Homo sapiens (9606) EEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHVSPLAIWLFISNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITAR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER68 TYR70 TYR87 HIS88 ARG91 GLY129 ASN130 PRO190 ASP191 TYR192 TRP212 GLY213 SER214 GLU215 THR216 TYR218 TYR259 TYR260 TYR261 GLY262 GLY263 TYR265 C: TYR157 ALA160 LYS161 ASP162 ARG163 PRO164 GLU165 ILE166 GLU168 VAL217 HIS218 PRO219 LYS220 GLY221 PRO222 LYS223 SER224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
