Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5208 |
| PDBID: | 7URC |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.14 |
| Reference: | 10.1038/s41586-022-04952-2 |
| Antibody | |
| Antibody: | 2C11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Isoform 2 of Protein-serine O-palmitoleoyltransferase porcupine |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7URC_H|Chain C[auth H]|2C11 heavy chain|Mus musculus (10090) MGWSCIILFLVATATGVHSEIQLQQSGAELVKPGASVKMSCKVSGYSFTGYNMNWVKQSHGKSLEWIGNINPYYVSTNYNQKFTGKATFTVDRSSSTAYMQLDSLTSEDSAVYYCARSYGSSHTFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: L
Mutation: NULL
| >7URC_L|Chain B[auth L]|2C11 light chain|Mus musculus (10090) MGWSCIILFLVATARTGVHSDIHMTQSPASLSAFVGETVTITCRTSENIFSYLAWYQQKQGKSPQLLVYNAKTLTSGVPSRFSGSGSGTQFSLKINSLQPEDFGSYYCQHHYGSPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7URC_A|Chain A|Isoform 2 of Protein-serine O-palmitoleoyltransferase porcupine|Homo sapiens (9606) MDYKDDDDKATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSCRWLQKVARSLALALLCLVLSTCVGPYLFPYFIPLNGDRLLRNKKRKARWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLU47 ASN48 PHE50 TYR52 ASN70 HIS111 TYR112 GLY113 SER114 TYR116 H: THR49 GLY50 TYR51 ASN52 ASN69 ASN71 TYR73 TYR74 SER76 THR77 ASN78 TYR119 GLY120 SER121 SER122 HIS123 A: SER49 HIS53 ARG90 HIS91 SER92 SER93 HIS94 ARG95 VAL97 PHE139 LEU141 ASP142 ARG143 GLY144 GLU145 VAL146 GLY147 THR148 VAL149 PRO150 GLU154 PHE173 HIS174 LEU177 GLN178 VAL180 GLN181 ARG183 GLU270 ASN315 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
