Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5204 |
| PDBID: | 7UR4 |
| Chains: | IJ_A |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.34 |
| Reference: | 10.1073/pnas.2203326119 |
| Antibody | |
| Antibody: | MPV467 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus (hMPV) fusion (F) protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >7UR4_I|Chain D[auth F], F[auth H], G[auth I]|MPV467 Fab Heavy chain|Homo sapiens (9606) LVQSGGGVVRPGTSLRVSCAAFDFNFRDYGMHWVRQAPGKGLEWVAGIWYDGSNKDYADSVKGRFTISRDNSQNTLYLQMNSLRVEDTAVYYCARDPRTHREGALSHFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKS |
Light Chain: J
Mutation: NULL
| >7UR4_J|Chain E[auth G], H[auth J], I[auth L]|MPV467 Fab Light chain|Homo sapiens (9606) ELTQDPAVSVALGQTVRITCQGDSLRNYFAGWYQQKPGQAPLLVLYGENIRPSGIPDRFSGSSSGNTVSLTITGAQAEDEADYYCNSRDNSGNHWVFGGGTRLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: A
Mutation: V84C/Q100R/S101R/L110C/T127C/A140C/A147C/N153C/D185P/L219K/V231I/A249C/N322C/T365C/E453Q/V463C
| >7UR4_A|Chain A, B, C|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTCSADQLAREEQIENPRRRRFVLGAIACGVATAAAVTAGVAIAKCIRLESEVTAIKNCLKKTNECVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISKDLMTDAELARAISNMPTSAGQIKLMLENRCMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPCEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPQDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: ASP26 PHE27 ASN28 ARG30 ASP31 TYR32 GLY33 TYR52 ARG94 PRO96 ARG97 THR98 HIS99 J: LEU28 ARG29 ASN30 TYR31 PHE32 TYR49 GLY50 GLU51 ILE53 ARG54 SER56 SER66 GLY68 A: TYR44 ALA136 CYS140 LYS143 THR144 ASN145 GLU146 CYS147 VAL148 SER149 THR150 VAL155 ARG156 VAL157 LEU158 ALA228 ARG229 SER232 ASN233 MET234 PRO235 THR236 SER237 ALA238 GLY239 ILE241 LYS242 LEU245 GLU246 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
