Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 520 |
| PDBID: | 3IU3 |
| Chains: | CD_J |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.4049/jimmunol.0903178 |
| Antibody | |
| Antibody: | Basiliximab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Basiliximab(Approved) |
| Antigen | |
| Antigen: | Interleukin-2 receptor alpha chain(CD25) |
| Antigen mutation: | No |
| Durg Target: | P01589 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >3IU3_C|Chain A, C, E[auth H]|Heavy chain of Fab fragment of Basiliximab|Mus musculus (10090) QLQQSGTVLARPGASVKMSCKASGYSFTRYWMHWIKQRPGQGLEWIGAIYPGNSDTSYNQKFEGKAKLTAVTSASTAYMELSSLTHEDSAVYYCSRDYGYYFDFWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEP |
Light Chain: D
Mutation: NULL
| >3IU3_D|Chain B, D, F[auth L]|Light chain of Fab fragment of Basiliximab|Mus musculus (10090) QIVSTQSPAIMSASPGEKVTMTCSASSSRSYMQWYQQKPGTSPKRWIYDTSKLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCHQRSSYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: J
Mutation: NULL
| >3IU3_J|Chain G[auth I], H[auth J], I[auth K]|Interleukin-2 receptor alpha chain|Homo sapiens (9606) ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLY24 TYR25 SER26 ARG29 TYR30 TRP31 HIS33 TRP45 ALA48 TYR50 ASN53 ASP55 SER57 TYR58 GLN60 GLU63 ASP97 TYR98 GLY99 TYR100 D: GLN1 SER30 TYR31 ASP49 LYS52 ARG90 SER91 SER92 TYR93 J: GLU1 LEU2 CYS3 ASP4 ASP5 ASP6 PRO7 GLU22 GLY23 MET25 ASN27 CYS28 GLU29 ILE37 LYS38 SER39 GLY40 SER41 LEU42 TYR43 LEU45 THR47 GLY48 ASN49 SER54 ASP56 ASN57 ILE118 TYR119 HIS120 PHE121 LYS153 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
