Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5196 |
| PDBID: | 7UPM |
| Chains: | B_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.str.2022.08.006 |
| Antibody | |
| Antibody: | 4.103 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human tribbles (TRIB2) pseudokinase |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7UPM_B|Chain B|Nb4.103 Nanobody|synthetic construct (32630) QVQLQESGGGLVQAGGSLRLSCAASGNISAQAYMGWYRQAPGKERELVAGISYGATTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAVISAGGGESIGYHFYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >7UPM_A|Chain A|Tribbles homolog 2|Homo sapiens (9606) GPGNLSHCVSCIGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFDISCYQESLAPCFCLSAHSNINQITEIILGETKAYVFFERSYGDMHSFVRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWFSTDFS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN31 TYR33 TYR37 GLU44 ARG45 GLU46 LEU47 SER52 TYR53 THR56 THR57 TYR58 ALA60 ASP61 LYS64 ILE98 SER99 ALA100 TYR108 PHE110 A: SER55 SER59 CYS60 GLY62 LYS63 LEU65 LEU66 LEU67 GLU68 ARG77 VAL79 LEU81 HIS82 SER83 GLY84 GLU86 ARG132 TYR134 GLU186 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
