Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5193 |
| PDBID: | 7UPK |
| Chains: | EG_B |
| Organism: | Henipavirus nipahense, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.80 |
| Reference: | 10.1038/s41467-023-36995-y |
| Antibody | |
| Antibody: | 1A9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Henipavirus nipahense fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7UPK_E|Chain B[auth C], E, H|Fab 1A9 heavy chain|Mus musculus (10090) QVQLQQSGAELVRPGTSVKISCKASGYTFTNYWLGWVKQRPGHGLEWIGDIYRGGGYTNYNEKFKGKATLTADTSSSTAYMQLSSLTSEDSAVYFCATRDGYFDYWGQGTTLTVSS |
Light Chain: G
Mutation: NULL
| >7UPK_G|Chain C[auth F], F[auth G], I[auth L]|Fab 1A9 light chain|Mus musculus (10090) DIQMTQSSSSFSVSLGDRTTITCKASEDIYNRLAWFQQKPGNAPRLLISGATSLETGVPSRFSGSGSGKDYTLSITSLQTEDVATYYCQQYWSSPWTFGGGTKLEIK |
Antigen
Chain: B
Mutation: NULL
| >7UPK_B|Chain A, D[auth B], G[auth D]|Fusion glycoprotein F0|Nipah henipavirus (3052225) MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNNTHDCVGDVRLAGVCMAGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQETAEKTVYVFTALQDYINTNLVPTIDKIPCKQTELSLDLALSKYLSDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVYFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYIKEAQRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR27 THR28 PHE29 THR30 ASN31 TYR32 TRP33 GLY53 GLY54 ASN58 ARG95 ASP96 GLY97 TYR98 ASP101 TYR102 G: ASP28 TYR30 ASN31 ARG32 SER49 GLY50 THR52 SER53 GLU55 THR56 TYR91 TRP92 SER93 TRP96 B: GLY41 VAL42 THR43 ARG44 LYS45 LYS47 ASN51 LEU53 LYS55 GLY247 TYR248 ALA249 THR250 GLU251 ASP252 LEU256 PHE282 PRO283 ILE284 THR286 GLU287 GLN289 GLN290 ALA291 TYR292 THR334 LYS335 ARG336 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
