Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5189 |
| PDBID: | 7UPD |
| Chains: | CF_G |
| Organism: | Henipavirus nipahense, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.40 |
| Reference: | 10.1038/s41467-023-36995-y |
| Antibody | |
| Antibody: | 2B12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Henipavirus nipahense fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7UPD_C|Chain B, C, H|Fab 2B12 heavy chain|Mus musculus (10090) MEFGLSWIFLAAILKGVQCEVQLVESGGGLVQPKGSLKLSCAASGFIFNTYAMHWVRQAPGKGLEWVARIRSKSSNYATYYADSVKDRFTISRDDSQSMLYLQMNNLKTEDTAMYYCVREGGYYFDYWGQGTTLTVSA |
Light Chain: F
Mutation: NULL
| >7UPD_F|Chain E, F, I[auth L]|Fab 2B12 light chain|Mus musculus (10090) MEFGLSWIFLAAILKGVQCDIVMSQSPSSLAVSVGEKVTMSCKSSQSLLHVSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYSYPWPFGGGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >7UPD_G|Chain A, D, G|Fusion glycoprotein F0|Nipah henipavirus (3052225) MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNNTHDCVGDVRLAGVCMAGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQETAEKTVYVFTALQDYINTNLVPTIDKIPCKQTELSLDLALSKYLSDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVYFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ILE28 ASN30 THR31 TYR32 ALA33 ARG50 SER52 SER53 GLU95 GLY97 TYR98 TYR99 ASP101 TYR102 F: SER27 ASN28 GLN29 LYS30 TYR32 TYR49 TRP50 THR53 SER56 TYR91 TYR92 G: ASN302 ASP304 ASN305 SER306 GLU307 VAL368 VAL369 SER370 SER371 ARG375 ILE388 SER389 VAL390 THR391 ARG399 ALA400 SER402 GLN403 SER404 GLY405 GLU406 GLN407 ASN439 SER440 GLY442 ILE443 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
