Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5182 |
| PDBID: | 7UOP |
| Chains: | BE_G |
| Organism: | Henipavirus nipahense, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.80 |
| Reference: | 10.1038/s41467-023-36995-y |
| Antibody | |
| Antibody: | 4H3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Henipavirus nipahense fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7UOP_B|Chain D[auth B], F[auth C], H|Fab 4H3 heavy chain|Mus musculus (10090) MEFGLSWIFLAAILKGVQCQIQLVQSGPELKKPGETVKISCKASGYTFRNYGVNWVKQGPGKDLKWMGWINTLNGEPTYADDFKRRFAFSLETSATTAFLQINNLKNEDTATYFCARTFYDGYYYAMDYWGQGTSVTVSA |
Light Chain: E
Mutation: NULL
| >7UOP_E|Chain E, G[auth F], I[auth L]|Fab 4H3 light chain|Mus musculus (10090) MEFGLSWIFLAAILKGVQCENVLTQSPTIMAASLGQKVTMTCSANSSVSSSYLHWYHQKSGASPKPLIHRTSNLASGVPARFIGSGSGTSFSLTISSVEAEDDATYYCQQWSGYPFITFGSGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >7UOP_G|Chain A, B[auth D], C[auth G]|Fusion glycoprotein F0|Nipah henipavirus (3052225) MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNNTHDCVGDVRLAGVCMAGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQETAEKTVYVFTALQDYINTNLVPTIDKIPCKQTELSLDLALSKYLSDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVYFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG30 ASN31 TYR32 GLY33 TRP50 THR52 LEU53 ASN54 GLU56 PHE96 TYR97 ASP98 GLY99 E: TYR32 ARG50 TYR94 G: LYS60 ILE62 PRO63 ASN64 VAL65 SER66 ASN67 SER69 GLN70 CYS71 THR72 GLY73 MET76 GLU77 LYS80 GLU152 ILE187 ASP188 PRO191 CYS192 LYS193 SER272 SER273 TYR274 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
