Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5176 |
| PDBID: | 7UNB |
| Chains: | HL_R |
| Organism: | Homo sapiens, Plasmodium falciparum |
| Method: | XRD |
| Resolution (Å): | 2.18 |
| Reference: | 10.1016/j.immuni.2022.07.015 |
| Antibody | |
| Antibody: | RUPA-117 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | malaria transmission-blocking antigen Pfs48/45-6C variant |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7UNB_H|Chain D[auth H]|RUPA-47 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKLSCTASGYTFTNFGITWVRQAPGQGLEWMAWISVYSGDTDYAQRFQGRVTMTTDTSTRTAYMEMRSLSFDDTAVYYCVRGGRDSPILGGYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >7UNB_L|Chain A[auth L]|RUPA-47 Fab kappa chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERVTLSCRASQSVRSMYLAWYQQKPGQAPRLLIHGASIRATGIPDRFSGSGSGTDFTLTISRLEREDFAVYYCHQYGSSPLTFGGGTKVESKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: R
Mutation: H18Y/G107L/I112V
| >7UNB_R|Chain B[auth R]|Gametocyte surface protein P45/48|Plasmodium falciparum (5833) EKKVIHGCNFSSNVSSKYTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFLIVGSVPKTTSFTCICKKDKKSAYMTVTIDSAGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ARG29 SER30 MET31 TYR32 ALA34 LEU46 HIS49 GLY50 SER52 ILE53 THR56 ASP60 TYR91 H: GLN1 VAL2 GLY26 TYR27 ASN31 PHE32 TYR53 ARG94 GLY95 GLY96 ARG97 ASP98 SER99 GLY100 GLY101 TYR102 R: ASP347 ILE348 ILE349 PRO350 ASP351 PHE354 GLN355 TYR357 GLN358 GLU360 GLU362 GLU363 LEU364 GLU365 PRO366 SER367 ASN368 ILE369 VAL370 TYR371 SER374 GLN375 ASN377 LYS404 ILE411 LYS413 LYS416 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
