Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5170 |
| PDBID: | 7UL7 |
| Chains: | GF_b |
| Organism: | Mammarenavirus lassaense, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.59 |
| Reference: | 10.1128/mbio.01278-22 |
| Antibody | |
| Antibody: | 18.5C-M30 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | prefusion GP (pfGP) ectodomain of arenavirus Lassa virus (LASV) LI (lineages I) G2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7UL7_G|Chain C[auth E], F[auth G], I[auth H]|18.5C-M30 Fab Heavy Chain|Homo sapiens (9606) METDTLLLWVLLLWVPGSTGDEVQLVQSGGGLVRPGGSLRLSCAAAGFTFKSYSMNWVRQAPGRGLEWVSSITSRGGSKTYYADVVKGRFTVSRDNAKQSLYLQMNSLRAEDTAIYFCARSRHSTSQPSYMDVWGRKITVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: F
Mutation: NULL
| >7UL7_F|Chain B[auth D], E[auth F], H[auth L]|18.5C-M30 Fab Light Chain|Homo sapiens (9606) METDTLLLWVLLLWVPGSTGDDIVLTQSPGTLSLSPGERATLSCRASQSVISYYVAWYQHKGGQAPRLLIYGASSRATGVPDRFSGSGSGTDFTLTISSLEPEDFALYYCQYYGSSPLWAFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: b
Mutation: NULL
| >7UL7_b|Chain J[auth a], K[auth b], L[auth c]|Glycoprotein G2|Lassa mammarenavirus (3052310) GTFTWTLSDSEGNETPGGYCLTRWMLIEAELKCFGNTAVAKCNEKHDEEFCDMLRLFDFNKQAIRRLKAPAQMSIQLINKAVNALINDQLIMKNHLRDIMCIPYCNYSKYWYLNHTSSGRTSLPKCWLISNGSYLNETQFSDDIEQQADNMITEMLQKEYLPETGLVDLEVDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: SER28 ILE30 SER31 TYR32 TYR33 TYR91 TYR92 GLY93 SER94 LEU97 G: THR52 SER53 ARG54 GLY55 SER57 LYS58 TYR60 ARG101 HIS102 SER103 THR104 SER105 b: ARG355 CYS359 ILE360 PRO361 LEU386 SER388 SER391 TYR392 LEU393 ASN394 GLU395 THR396 GLN397 PHE398 SER399 ASP400 ASP401 ILE402 GLU403 GLN404 ASP407 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
