Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5167 |
| PDBID: | 7UL2 |
| Chains: | D_R |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 2.40 |
| Reference: | 10.1038/s41594-022-00859-8 |
| Antibody | |
| Antibody: | Nb6 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human neurotensin receptor 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7UL2_D|Chain B[auth D]|Nanobody 6|Lama glama (9844) MAQVQLQESGGGLVQAGESLRLSCAASGTIFRLYDMGWYRRVSGNQRELVASITSGGSTKYGDSVKGRFTISRDNAKNTVYLQMSSLKPEDTAVYYCNAEYRTGIWEELLDGWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: R
Mutation: NULL
| >7UL2_R|Chain A[auth R]|Neurotensin receptor 1|Homo sapiens (9606) DYKDDDDAMGQPGNGSAFLLAPNRSHAPDHDVENLYFQGQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTLFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLYTLMILRLKSVRLLSGSREKDRNLRRITRLVLAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: PHE31 ARG32 TYR34 ASP35 GLU100 ARG102 ILE105 TRP106 GLU107 GLU108 LEU109 LEU110 ASP111 R: TYR256 ILE260 LEU263 LYS264 SER265 VAL266 ARG267 LEU268 ARG273 ASP295 ARG296 ARG299 ARG300 THR302 ARG303 LEU306 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
