Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5165 |
| PDBID: | 7UL0 |
| Chains: | HL_A |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.49 |
| Reference: | 10.1016/j.isci.2022.105783 |
| Antibody | |
| Antibody: | EH8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7UL0_H|Chain B[auth H]|Heavy chain of EH8|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFSSYGISWVRQAPGQGLEWMGWISPYNGNTKYPQKFQGRVTMTTDTSTNTAYMELRSLRSDDTAVYYCARDLELGGGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >7UL0_L|Chain C[auth L]|Light chain of EH8|Homo sapiens (9606) QSALTQPASVSGSPGQSITISCTGTSSDVGSYNLVSWYQQHPDKAPKFMIYEGTKRPSGVSNRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGNSTWVFGGGTKLTVLRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7UL0_A|Chain A|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 SER31 TYR32 TRP50 TYR53 ASN54 ASN56 LEU96 GLU97 LEU98 GLY99 L: TYR30 LEU32 GLU50 TYR91 ASN94 TRP96 A: ALA475 GLY476 SER477 THR478 PRO479 CYS480 ASN481 VAL483 GLU484 GLY485 PHE486 ASN487 CYS488 TYR489 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
