Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5163 |
| PDBID: | 7UIJ |
| Chains: | HL_C |
| Organism: | Mus musculus, Borreliella burgdorferi B31 |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1128/mbio.02981-22 |
| Antibody | |
| Antibody: | B5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Borreliella burgdorferi B31 outer surface protein C (OspC) type A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7UIJ_H|Chain A, C[auth H]|Monoclonal B5 Fab Heavy Chain|Mus musculus (10090) EVQLEESGPEQKKPGETVMMSCKASGYTFTDHSMHWVKQAPGKGLKWMGWINTETGEPTCADDFKGRFALSLETSSRTAFLQINNLKNEDTAIYFCIGRWGYYVYWGQGTTLVVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGG |
Light Chain: L
Mutation: NULL
| >7UIJ_L|Chain B, D[auth L]|Monoclonal B5 Fab Light Chain|Mus musculus (10090) DIQMTQSPASLSVSVGESVTIICRASENINSNLAWYQQKQGKPPQLLVYAATNLAGGVPSRFSGSGSGTQYSLKINSLQSEDFGTYYCQHVWGSPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >7UIJ_C|Chain E[auth C], F[auth D]|Outer surface protein C|Borreliella burgdorferi B31 (224326) KGPNLTEISKKITDSNAVLLAVKEVEALLSSIDEIAAKAIGKKIHQNNGLDTENNHNGSLLAGAYAISTLIKQKLDGLKNEGLKEKIDAAKKCSETFTNKLKEKHTDLGKEGVTDADAKEAILKTNGTKTKGAEELGKLFESVEVLSKAAKEMLANSVKELTSP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 GLY26 TYR27 THR28 THR30 HIS32 GLU54 ARG77 TRP100 GLY101 TYR102 TYR103 TYR105 L: ASN30 SER31 ASN32 TYR49 ALA50 THR52 ASN53 LEU54 GLY56 SER67 C: GLU63 ASP70 GLU71 ALA73 ALA74 LYS75 LYS79 ASN85 LEU87 LYS156 LEU160 LYS161 THR162 ASN163 GLY164 LYS166 GLU171 GLY174 LYS175 PHE177 GLU178 GLU181 LYS185 LYS188 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
