Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5149 |
| PDBID: | 7UE9 |
| Chains: | HL_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.75 |
| Reference: | 10.3389/fimmu.2022.869725 |
| Antibody | |
| Antibody: | 3d8b Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Complement C3dg fragment |
| Antigen mutation: | No |
| Durg Target: | P01024 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7UE9_H|Chain B[auth H]|Fab heavy chain|Mus musculus (10090) MGSTAILGLLLAVLQGVCAQVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYINWVRQAPGQGLEWMGVINPYSGGTSYNQKFKGRVTMTVDTSTSTAYMELSSLRSEDTAVYFCSSPYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPPCPAPEFEGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVLHEALHSHYTQKSLSLSLG |
Light Chain: L
Mutation: NULL
| >7UE9_L|Chain A[auth L]|Fab light chain|Mus musculus (10090) MDMRVPAQLLGLLLLWLRGARCDVVMTQSPLSLPVTLGQPASISCKSSQSLLDSDGKTYLNWFQQRPGQSPRRLIYLVSKLDSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCWQGTHFPRTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7UE9_C|Chain C|Complement C3dg fragment|Homo sapiens (9606) GAVDAERLKHLIVTPSGAGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP31 SER32 ASP33 LYS35 TYR37 ASN39 ARG51 TYR54 LEU55 LYS58 LEU59 ASP60 SER61 GLY62 TRP94 GLY96 THR97 PHE99 ARG101 H: THR28 ASN31 TYR32 TYR33 ASN35 VAL50 ASN52 SER55 GLY57 THR58 SER59 TYR60 GLN62 LYS65 SER97 SER98 PRO99 TYR100 TRP101 C: GLY0 ASP254 ARG261 ASN264 ASP295 HIS296 GLN297 GLU298 LEU299 ASN300 LEU301 ASP302 VAL303 SER304 LEU305 GLN306 LEU307 PRO308 SER309 ARG310 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
