Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5147 |
| PDBID: | 7UDS |
| Chains: | DE_B |
| Organism: | Homo sapiens, Mammarenavirus lassaense |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1128/mbio.01278-22 |
| Antibody | |
| Antibody: | 25.10C Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | prefusion GP (pfGP) ectodomain of arenavirus Lassa virus (LASV) LI (lineages I) G1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7UDS_D|Chain A[auth D], B[auth F], D[auth H]|25.10C Fab Heavy Chain|Homo sapiens (9606) QVQLQESGGGLVKPGGSLRLSCTASGFNFNKYNMNWVRQAPGKGLEWVSSISALSTYIYYADSLKGRFTVSRDNAKNSLFLQMNSLRDDDTAVYYCAREIRRASTWSADLWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: E
Mutation: NULL
| >7UDS_E|Chain C[auth E], E[auth G], F[auth L]|25.10C Fab Light Chain|Homo sapiens (9606) IQMTQSPSSLSASVGDRVIITCRASQSISSSLNWYQQKPGKAPKLLIYAAVNLETGVPSRFSGSGFGTDFTLAISNVQPEDFATYYCQQSDTRTFGRGTKLDVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: B
Mutation: NULL
| >7UDS_B|Chain G[auth A], H[auth B], I[auth C]|Glycoprotein G1|Lassa mammarenavirus (3052310) MGQIITFFQEVPHVIEEVMNIVLIALSLLAILKGLYNIATCGIIGLVAFLFLCGKSCSLTLKGGYELQTLELNMETLNMTMPLSCTKNSSHHYIRVGNETGLELTLTNTSIINHKFCNLSDAHKKNLYDHALMSIISTFHLSIPNFNQYEAMSCDFNGGKISVQYNLSHSYAGDAAEHCGTVANGVLQTFMRMAWGGRYIALDSGCGNWDCIMTSYQYLIIQNTTWEDHCQFSRPSPIGYLGLLSQRTRDIYISRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ASN30 LYS31 TYR32 SER52 ALA53 LEU54 THR56 TYR57 ARG101 SER104 TRP106 B: ASN73 GLU75 LYS87 ASN98 GLU99 THR100 THR224 THR225 TRP226 GLU227 ASP228 HIS229 GLN231 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
