Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 514 |
| PDBID: | 3I50 |
| Chains: | HL_E |
| Organism: | West Nile virus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/emboj.2009.245 |
| Antibody | |
| Antibody: | E53 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | West Nile Virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3I50_H|Chain C[auth H]|murine heavy chain (IgG3) of E53 monoclonal antibody Fab|Mus musculus (10090) EVKLVESGGGLVLPGGSLRLSCATSGFTFTDYYMTWVRQPPGKALEWLGFIGNKANDYTTEYSASVKGRFTISRDDSQSILYLQMSTLRAEDRATYYCATVYGNYPYFDVWGVGTTVAVSSATTTAPSVYPLVPGCSDTSGSSVTLGCLVKGYFPEPVTVKWNYGALSSGVRTVSSVLQSGFYSLSSLVTVPSSTWPSQTVICNVAHPASKVDLIKEISGP |
Light Chain: L
Mutation: NULL
| >3I50_L|Chain B[auth L]|murine kappa light chain of E53 monoclonal antibody Fab|Mus musculus (10090) QIVLTRTGGIMSASPGEKVTMTCSASSSVSYMHWYQQKSGTSPKIWIYESSKLASGVPVRFSGSGSGTSYSLTISSMEAEDVATYYCQQWSSHPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKS |
Antigen
Chain: E
Mutation: NULL
| >3I50_E|Chain A[auth E]|Envelope glycoprotein|West Nile virus (11082) FNCLGMSNRDFLEGVSGATWVDLVLEGDSCVTIMSKDKPTIDVKMMNMEAANLAEVRSYCYLATVSDLSTKAACPTMGEAHNDKRADPAFVCRQGVVDRGWGNGCGLFGKGSIDTCAKFACSTKAIGRTILKENIKYEVAIFVHGPTTVESHGNYSTQVGATQAGRFSITPAAPSYTLKLGEYGEVTVDCEPRSGIDTNAYYVMTVGTKTFLVHREWFMDLNLPWSSAGSTVWRNRETLMEFEEPHATKQSVIALGSQEGALHQALAGAIPVEFSSNTVKLTSGHLKCRVKMEKLQLKGTTYGVCSKAFKFLGTPADTGHGTVVLELQYTGTDGPCKVPISSVASLNDLTPVGRLVTVNPFVSVATANAKVLIELEPPFGDSYIVVGRGEQQINHHWHKSGS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TRP91 SER92 H: ASP31 TYR32 TYR33 PHE50 ALA52 ASN53 VAL95 TYR96 GLY97 ASN98 TYR99 E: CYS74 PRO75 THR76 MET77 GLY78 GLU79 VAL96 TRP101 GLY104 CYS105 GLY106 LEU107 GLY109 LYS110 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
