Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5139 |
| PDBID: | 7UCF |
| Chains: | DE_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 4.00 |
| Reference: | 10.1126/sciadv.abp8155 |
| Antibody | |
| Antibody: | BG24 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP.664 envelope glycoprotein- gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7UCF_D|Chain B[auth D]|BG24 Fab heavy chain|Homo sapiens (9606) QVQLVQSRAEVKKPGASVKVSCEASGYNFVDHYIHWVRQAPGQRPQWVGWMNPRGGGVNYSQRFQGRVTMTRDTSIDTAYMQLNRLTSGDTAVYYCATQVKLDSSAGYPFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKHHHHHH |
Light Chain: E
Mutation: NULL
| >7UCF_E|Chain C[auth E]|BG24 light chain|Homo sapiens (9606) QSALTQPRSVSGSPGQSVNISCTGAYSGLGWYQQHPGRAPKLIIYEVNRRPSGVSDRFSGSKSGNTASLTISGLRTEDEADYFCSAFEYFGEGTKLTVLSQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: NULL
| >7UCF_G|Chain D[auth G]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) MDAMKRGLCCVLLLCGAVFVSPAGAGENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: VAL30 ASP31 TYR33 HIS35 TRP47 TRP50 ASN52 ARG54 GLY55 GLY56 GLY57 VAL58 ASN59 TYR60 SER61 GLN62 ARG63 GLN65 ARG72 THR74 SER105 ALA106 GLY107 TYR108 E: ALA3 TYR26 PHE87 GLU88 TYR89 G: LYS97 HIS105 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 ASN283 ARG360 SER365 GLY366 GLY367 ASP368 VAL371 TRP427 GLN428 ILE430 THR455 ARG456 ASP457 GLY458 GLY459 SER460 THR461 ASN462 SER463 THR465 THR467 ARG469 GLY473 ASP474 ARG476 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
