Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5124 |
| PDBID: | 7U9G |
| Chains: | HE_C |
| Organism: | Rabies virus strain Pasteur vaccin, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.39 |
| Reference: | 10.1126/sciadv.abp9151 |
| Antibody | |
| Antibody: | RVA122 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rabies virus glycoprotein (RABV-G) pre-fusion ectodomain trimer |
| Antigen mutation: | No |
| Durg Target: | P08667 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7U9G_H|Chain G, H, I|RVA122 Fab Heavy Chain|Homo sapiens (9606) QVHLQESGPGLVKPSETLSLTCTVSGDSMNNFYWGWIRQPAGKGLEWIGYIYYSGTTNYNPSLKSRVTISIDTSKNQFSLKVNSVTAADTAVYYCARDSGDYVSYYYYGMDVWGPGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDLEVDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Light Chain: E
Mutation: NULL
| >7U9G_E|Chain D, E, F|RVA122 Fab Light Chain|Homo sapiens (9606) QSVLTQSPSASDTPGQRVTISCSGSSSNIGSNYVYWYQQFPGTAPKLLIYKSDKRPSGVPDRFSGSTSGTSASLAISGLRSEDEADYYCAAWDNRLSGWLFGGGTKLTVLGTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7U9G_C|Chain A, B, C|Glycoprotein|Rabies virus (103929) MVPQALLFVPLLVFPLCFGKFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYISAIKMNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYHWLRTVKTTKESLVIISPSVADLDPYDRSLHSRVFPGGNCSGVAVSSTYCSTNHDYTIWMPENPRLGMSCDIFTNSRGKRASKGSETCGFVDERGLYKSLKGACKLKLCGVLGLRLMDGTWVAMQTSNETKWCPPGQLVNLHDFRSDEIEHLVVEELVKKREECLDALESIMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEIIPSKGCLRVGGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSLLQQHMELLVSSVIPLMHPLADPSTVFKNGDEAEDFVEVHL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ILE29 GLY30 SER31 ASN32 TYR33 TYR50 LYS51 LYS54 SER57 THR67 TRP92 ASN94 ARG95 SER97 TRP99 H: ASP244 ASN248 TYR250 TYR267 TYR269 TYR270 SER271 THR273 THR274 ASN275 ASP315 GLY317 ASP318 TYR319 VAL320 SER321 TYR322 TYR323 TYR324 TYR325 C: LYS1 PHE2 PRO3 ILE4 VAL29 GLU31 GLU33 CYS35 THR36 LEU38 LYS198 ALA200 VAL210 GLU212 ARG213 GLY214 LEU215 TYR216 VAL308 PRO309 LYS330 SER331 ARG333 THR334 ASN336 GLU337 ARG346 SER379 LEU380 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
