Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 511 |
| PDBID: | 3HI6 |
| Chains: | XY_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1073/pnas.0909301106 |
| Antibody | |
| Antibody: | AL-57 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Integrin alpha-L |
| Antigen mutation: | No |
| Durg Target: | P20701 |
Sequence information
Antibody
Heavy Chain: X
Mutation: NULL
| >3HI6_X|Chain C[auth H], E[auth X]|Heavy chain of Fab fragment of AL-57 against alpha L I domain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSRYVMWWVRQAPGKGLEWVSYIWPSGGNTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCASSYDFWSNAFDIWGQGTMVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVES |
Light Chain: Y
Mutation: NULL
| >3HI6_Y|Chain D[auth L], F[auth Y]|light chain of Fab fragment of AL-57 against alpha L I domain|Homo sapiens (9606) QDIQMTQSPSSLSASVGDRVTITCRASQSIGSYLNWYQQKTGKAPKALIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQLEDFATYYCQQSYSTPSFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: B
Mutation: NULL
| >3HI6_B|Chain A, B|Integrin alpha-L|Homo sapiens (9606) GNVDLVFLFDGSMSLQPDEFQKILDFMKDVMKKCSNTSYQFAAVQFSTSYKTEFDFSDYVKWKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLCTELQKKIY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| X: SER30 ARG31 TYR32 VAL33 TRP52 PRO53 SER54 ASN57 ASN74 TYR100 ASP101 PHE102 TRP103 SER104 ASN105 ALA106 Y: SER31 TYR32 ASN34 TYR49 ALA50 SER52 SER53 GLN55 GLY66 SER67 SER91 TYR92 B: MET140 SER141 LEU142 GLN143 PRO144 ASP145 LYS200 HIS201 MET202 LEU203 LEU204 LEU205 THR206 ASP239 GLY240 GLU241 ALA242 THR243 ASP244 LYS263 HIS264 THR267 GLU269 THR273 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
