Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5108 |
| PDBID: | 7U8G |
| Chains: | DE_A |
| Organism: | Homo sapiens, Oryctolagus cuniculus |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/s41467-022-33711-0 |
| Antibody | |
| Antibody: | 7G5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 2 |
| Antigen mutation: | No |
| Durg Target: | P04839 |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7U8G_D|Chain C[auth D]|7G5 - heavy chain|Oryctolagus cuniculus (9986) QSLEESGGDLVKPGASLTLTCTASGIDFSGYHYMCWVRQAPGKGLEWIGCTHSGDGTTYYARWAKGRFTISKTSSTTVTLQMTSLTAADTATYFCARRYVFSGGYSGLDSWGPGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: E
Mutation: NULL
| >7U8G_E|Chain D[auth E]|7G5 - light chain|Oryctolagus cuniculus (9986) ALVMTQTPSSVSAAVRGTVTIKCQASENIYSNLAWYQQKPGQPPKLLIYGASKLASGVPSRFKGSGSGTDYTLTIRDLEAADAATYYCQQFYDSLNTDNAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7U8G_A|Chain A|EGFP, Cytochrome b-245 heavy chain chimera|Homo sapiens (9606) MSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKGSGMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGENLYFQGGSGLVPRGSGSGGNWAVNEGLSIFVILVWLGLNVFLFVWYYRVYDIPPKFFYTRKLLGSALALARAPAACLNFNCMLILLPVCRNLLSFLRGSSACCSTRVRRQLDRNLTFHKMVAWMIALHSAIHTIAHLFNVEWCVNARVNNSDPYSVALSELGDRQNESYLNFARKRIKNPEGGLYLAVTLLAGITGVVITLCLILIITSSTKTIRRSYFEVFWYTHHLFVIFFIGLAIHGAERIVRGQTAESLAVHNITVCEQKISEWGKIKECPIPQFAGNPPMTWKWIVGPMFLYLCERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFFSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASILKSVWYKYCNNATNLKLKKIYFYWLCRDTHAFEWFADLLQLLESQMQERNNAGFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAETLSKQSISNSESGPRGVHFIFNKENF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR31 TYR33 HIS52 GLY54 ASP55 THR57 TYR59 ARG98 VAL100 PHE101 SER102 GLY103 GLY104 TYR105 SER106 E: GLU27 TYR30 ASN32 PHE91 TYR92 ASP93 SER94 LEU95 ASN96 A: THR242 VAL243 GLU245 GLN246 LYS247 SER249 GLU250 GLY252 LYS253 ILE254 LYS255 GLU256 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
