Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5099 |
| PDBID: | 7TZH |
| Chains: | A_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.43 |
| Reference: | 10.1038/s41590-022-01238-7 |
| Antibody | |
| Antibody: | F7 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human lymphocyte activation gene 3 (LAG3) domains 3 and 4 |
| Antigen mutation: | No |
| Durg Target: | P18627 |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >7TZH_A|Chain A, C|scFvF7|Homo sapiens (9606) MAQVQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMNWVRQAPGKGLEWVSGISAGGTGTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDQDGGKKTDAFDIWGQGTMVTVSSGGGGSGGGGSGGGGSDIVMTQSPSLLSASVGDRVTITCRASQGINYYLAWYQQKPGKAPKLLIYAASTLQSGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCQQTGYYPLTFGPGTKVDIAA |
Antigen
Chain: B
Mutation: NULL
| >7TZH_B|Chain B, D|Lymphocyte activation gene 3 protein|Homo sapiens (9606) GLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR58 GLY59 THR60 TYR61 LYS67 ASP101 GLY104 GLY105 LYS106 LYS107 ASP109 PHE111 SER139 ASP140 ILE141 GLN166 GLY167 ASN169 GLY207 THR208 THR230 GLY231 TYR232 TYR233 PRO234 LEU235 B: ARG379 PHE380 VAL381 PRO388 SER389 GLN390 ARG391 SER392 PHE393 SER394 GLY395 PRO396 GLU399 GLN404 GLU418 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
