Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5090 |
| PDBID: | 7TXZ |
| Chains: | EF_A |
| Organism: | Henipavirus nipahense, Mus sp. |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1126/science.abm5561 |
| Antibody | |
| Antibody: | nAH1.3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nipah Virus attachment (G) glycoprotein ectodomain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7TXZ_E|Chain C[auth E], E[auth H]|nAH1.3 Fab heavy chain|Mus sp. (10095) EVKLEESGGGLVQPGGSMKLSCVASGFSFSYYWMNWVRQSPEKGLEWVAEIRLKSNNYGTHYAESVKGRFTISRDDSKSSVYLQMNNLRPEDTGIYYCTRVITTVFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGKGGSGGGSWSHPQFEK |
Light Chain: F
Mutation: NULL
| >7TXZ_F|Chain D[auth F], F[auth L]|nAH1.3 Fab light chain|Mus sp. (10095) DIVLTQSPASLAVSLGQRATISCRASESVHDYGISFMNWFQQKPGQPPKLLIYSASNQGSGVPARFSGSGSGTDFSLNIHPMEEDDIAMYFCQQSKEVPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7TXZ_A|Chain A, B, G[auth C], H[auth D]|Glycoprotein G|Nipah henipavirus (3052225) HHHHHHIQNYTRSTDNQAVIKDALQGIQQQIKGLADKIGTEIGPKVSLIDTSSTITIPANIGLLGSKISQSTASINENVNEKCKFTLPPLKIHECNISCPNPLPFREYRPQTEGVSNLVGLPNNICLQKTSNQILKPKLISYTLPVVGQSGTCITDPLLAMDEGYFAYSHLERIGSCSRGVSKQRIIGVGEVLDRGDEVPSLFMTNVWTPPNPNTVYHCSAVYNNEFYYVLCAVSTVGDPILNSTYWSGSLMMTRLAVKPKSNGGGYNQHQLALRSIEKGRYDKVMPYGPSGIKQGDTLYFPAVGFLVRTEFKYNDSNCPITKCQYSKPENCRLSMGIRPNSHYILRSGLLKYNLSDGENPKVVFIEISDQRLSIGSPSKIYDSLGQPVFYQASFSWDTMIKFGDVLTVNPLVVNWRNNTVISRPGQSQCPRFNTCPEICWEGVYNDAFLIDRINWISAGVFLDSNQTAENPVFTVFKDNEILYRAQLASEDTNAQKTITNCFLLKNKIWCISLVEIYDTGDNVIRPKLFAVKIPEQCY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLU1 GLY26 PHE27 SER28 TYR31 TYR32 LEU53 ILE102 THR103 THR104 VAL105 ALA107 TYR108 F: ASP31 TYR32 GLY33 ILE34 SER35 PHE36 LEU50 TYR53 SER54 SER56 ASN57 GLN58 GLY59 SER60 GLY61 ALA64 A: ARG172 THR175 GLY183 LEU184 PRO185 ASN186 ASN187 ILE188 LEU190 GLN191 GLN358 LEU448 GLY449 GLN450 PRO451 VAL469 LEU470 VAL472 ASN478 ASP515 ARG516 ILE517 ASN518 LYS541 ASN570 LYS571 GLU599 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
