Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5088 |
| PDBID: | 7TUG |
| Chains: | HL_D |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.90 |
| Reference: | 10.1038/s41467-022-33153-8 |
| Antibody | |
| Antibody: | PaSta2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human tapasin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7TUG_H|Chain B[auth H]|PaSta2 Fab heavy chain|Mus musculus (10090) QVQLQQSGAELVRPGTSVKMSCKAAGYTFTNYWIGWIKQRPGHGLEWIGDIYPGGGYTNYNEKFKVKATLTADTSSVTAYMQLSSLTSEDSAIYYCTREGNYGFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKGSHHHHHH |
Light Chain: L
Mutation: NULL
| >7TUG_L|Chain C[auth L]|PaSta2 Fab kappa light chain|Mus musculus (10090) DIVMTQSPSSLAMSVGQKVTLSCKSSQSLLNSINQKNYLAWYQQKPGQSPKMLVYFASTRESGVPDRFIGSGSGTDFTLTISSVQAEDLADYFCQQHYSTPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >7TUG_D|Chain A[auth D]|Tapasin|Homo sapiens (9606) GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEGGLEVLFQGPGGGLNDIFEAQKIEWHEGGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLN1 ASN31 TYR32 TRP33 ASP50 TYR52 GLY56 ASN59 GLU99 GLY100 ASN101 TYR102 TYR106 L: LEU30 ASN31 SER32 ILE33 ASN34 GLN35 TYR38 TYR55 THR59 ARG60 GLU61 SER62 GLY63 ASP66 HIS97 TYR98 SER99 THR100 LEU102 D: HIS70 SER109 SER110 PRO111 SER114 PRO146 ALA147 ARG188 HIS190 LEU191 LYS193 GLY194 HIS195 GLU213 PRO243 PHE244 GLN245 GLU246 GLY247 THR248 THR263 LEU264 GLU265 ALA267 SER303 GLY304 GLY305 LEU306 GLU307 SER330 LEU332 HIS334 VAL340 LEU342 PRO367 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
