Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5086 |
| PDBID: | 7TUF |
| Chains: | AB_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1038/s41467-022-33153-8 |
| Antibody | |
| Antibody: | PaSta1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human tapasin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7TUF_A|Chain B[auth A], E[auth H]|PaSta1 Fab heavy chain|Mus musculus (10090) DVQLQESGPGLVIPSQSLSLTCTVTGYSITTDYAWNWIRQFPGNRLEWMGYISSSGVTVYNPSLKSRISITRDTSKNQFFLQLISVTTEDTATYYCARRGYYRYDSIDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKGSHHHHHH |
Light Chain: B
Mutation: NULL
| >7TUF_B|Chain C[auth B], F[auth L]|PaSta1 Fab kappa light chain|Mus musculus (10090) QIVLSQSPAILSASPGEKVTMTCRATSSVSYIHWYQQKPGSSPKPWIYATSSLTSGVPVRFSGSGSGTSYSLTISRVEAEDAATYYCQQWSSNPPTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >7TUF_C|Chain A[auth C], D|Tapasin|Homo sapiens (9606) GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEGGLEVLFQGPGGGLNDIFEAQKIEWHEGGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR31 ASP32 ALA34 TYR51 SER53 SER54 SER55 VAL57 THR58 VAL59 ARG99 GLY100 TYR101 TYR102 ARG103 TYR104 B: SER28 VAL29 SER30 TRP90 SER91 SER92 ASN93 C: PRO51 MET165 PRO166 PRO167 THR168 ALA171 ALA172 SER173 SER174 LEU175 ALA176 PRO177 ASP223 ASP224 GLU225 PRO226 TRP227 GLY228 PRO229 TRP230 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
