Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5081 |
| PDBID: | 7TRH |
| Chains: | HB_G |
| Organism: | Influenza A virus (A/reassortant/NYMC X-181(California/07/2009 x NYMC X-157)(H1N1)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1371/journal.pbio.3002415 |
| Antibody | |
| Antibody: | K03.28 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus reassortant/NYMC X-181(California/07/2009 x NYMC X-157) H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7TRH_H|Chain B[auth H]|K03.28 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLIQPGGSLRLSCEASAFTFSSYEMNWVRQAPGKGLEWVSYITSSGSRIYYADSVKGRFTISRDNAKNSLYLQMNSLRVEDTAVYYCARLLDSIVWGEGWYYGMDVWGQGTTVTVSGASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKHHHHHH |
Light Chain: B
Mutation: NULL
| >7TRH_B|Chain C[auth B]|K03.28 Fab lambda light chain|Homo sapiens (9606) ASSYELTQSPSVSVAPGRTARITCGGNDIGLKGVHWYQQKPGQAPVLVLYDNNHRPSGIPERFSGSISGDTATLTVTRVEADDGADYFCQVWDTSSGPPHVIFGGGTKLTVLSQPKGAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: NULL
| >7TRH_G|Chain A[auth G]|Hemagglutinin|Influenza A virus (673446) ASAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSDKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADAYVFVGSSRYSKTFKPEIAIRPKVRDREGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 ARG57 ASP101 SER102 ILE103 VAL104 TRP105 GLY106 GLU107 GLY108 TRP109 TYR110 TYR111 B: GLY28 LEU29 LYS30 ASP49 ASN50 HIS52 ILE65 GLY67 G: TYR98 LYS133 GLY134 VAL135 THR136 ALA137 PRO140 ALA142 GLY143 ALA144 LYS145 TRP153 VAL155 LYS156 ASN159 HIS183 PRO185 SER186 THR187 ALA189 ASP190 SER193 LEU194 ASP225 ARG226 GLU227 GLY228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
