Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5074 |
| PDBID: | 7TPJ |
| Chains: | HL_B |
| Organism: | Cupriavidus metallidurans, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.46 |
| Reference: | 10.1038/s41586-022-04555-x |
| Antibody | |
| Antibody: | WaB10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Putative cell surface polysaccharide polymerase/ligase apo-CmWaaL |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7TPJ_H|Chain B[auth H]|Fab Heavy (H) Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVYSSSIHWVRQAPGKGLEWVAYISSYYGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARIMFKWVSPNMAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC |
Light Chain: L
Mutation: NULL
| >7TPJ_L|Chain C[auth L]|Fab Light (L) Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSYYSLVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7TPJ_B|Chain A[auth B]|Putative cell surface polysaccharide polymerase/ligase|Cupriavidus metallidurans (119219) MTDSNKLLSRMATVLVFAFPVLILCVPRGAGVFLAGVGVLALLGWRGMGRAWREYSKVMTPLAIAVLAFMLVYVGSKLYFHTPWNVIDNPSRTLLAILTCWVIVRAAPNPAWLWRGITVGLFLALLIVGYQKFALNIDRPSAWIQAIAFANMIAALALVGFARPGDSRGTHMEAWVNLLLGTMILMLNGTRGAVVAMLVTSVPMLMIRYRRFSVRMLIVAVCAVATLAIGAYMVPDSPVSKRVDDAVSEIQMYRQGNIETSVGVRLKIWHIGLQYFSEHPWTGVGVGQFARILHASEFCHETKSLACVLEHAHNDIVEAASTTGIPGLMVMLGLFLVPAVLFARALRAARSLGNPQGVSLGGAGLGVVMASLISGLTQVTMAHQANVVFYAGLIGLLLGMAGREAHSARTDAV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL5 GLY29 PHE30 ASN31 TYR33 SER34 TYR53 TYR57 TYR58 TYR62 THR77 ARG101 MET103 PHE104 LYS105 TRP106 VAL107 PRO109 ASN110 MET111 TYR115 L: SER31 SER32 TYR50 LEU55 TYR56 SER57 GLY58 ARG67 SER92 TYR93 TYR94 SER95 LEU96 B: GLN131 LYS132 LEU135 ASN136 ILE137 ASP138 ARG139 SER141 ALA142 GLN145 ALA146 ILE147 MET186 THR190 ARG191 GLY192 LYS241 ARG242 ASP244 ASP245 SER248 GLU249 GLU259 THR260 SER261 VAL264 ARG265 ILE268 LYS303 LEU305 VAL308 LEU309 HIS313 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
