Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5069 |
| PDBID: | 7TN9 |
| Chains: | GH_T |
| Organism: | Mus musculus, Ebola virus - Mayinga, Zaire, 1976 |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1016/j.chom.2023.01.002 |
| Antibody | |
| Antibody: | REGN 3470 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus envelope glycoprotein GP1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; Q05320; Q05320; Q05320; |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7TN9_G|Chain K[auth G], Q[auth I]|REGN3470 heavy chain|Mus musculus (10090) QVQLVESGGGVVQPGRSLRLSCAASGFTFNNYGMHWVRQAPGMGLEWVAVIWHDGSDKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARNWNLFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVE |
Light Chain: H
Mutation: NULL
| >7TN9_H|Chain L[auth H], R[auth J]|REGN3470 light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRITITCRASQSISTYLHWYQQKPGKAPKLLIYAASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSFSTPPINFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: T
Mutation: NULL
| >7TN9_T|Chain B[auth S], C[auth T], D[auth U]|Envelope glycoprotein|Ebola virus - Mayinga, Zaire, 1976 (128952) RSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRRE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: GLY26 PHE27 THR28 ASN31 TYR32 GLY33 TRP52 HIS53 ASP54 SER56 ASP57 TYR59 ARG98 ASN99 TRP100 ASN101 LEU102 H: SER30 THR31 TYR32 HIS34 TYR49 ALA50 THR53 PHE92 SER93 THR94 PRO95 T: GLU245 ARG247 THR259 THR262 SER263 LYS265 LYS276 VAL277 ASN278 PRO279 GLU280 ILE281 ASP282 THR283 THR284 ARG302 SER303 GLU304 GLU305 LEU306 SER307 PHE308 THR309 VAL310 ASN312 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
