Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5065 |
| PDBID: | 7TN9 |
| Chains: | EF_S |
| Organism: | Mus musculus, Ebola virus - Mayinga, Zaire, 1976 |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1016/j.chom.2023.01.002 |
| Antibody | |
| Antibody: | REGN 3471 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus envelope glycoprotein GP1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; Q05320; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7TN9_E|Chain A, S[auth C], T[auth E]|REGN3471 heavy chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYDMHWVRQATGKGLEWVSAIGTAGDTYYPGSVKGRFTISRENAKNSLYLQMNSLRAGDTAVYYCARTWFGELYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVE |
Light Chain: F
Mutation: NULL
| >7TN9_F|Chain G[auth B], U[auth D], V[auth F]|REGN3471 light chain|Mus musculus (10090) DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTEFTLTITSLQAEDVAVYYCQQYYSSPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: S
Mutation: NULL
| >7TN9_S|Chain B[auth S], C[auth T], D[auth U]|Envelope glycoprotein|Ebola virus - Mayinga, Zaire, 1976 (128952) RSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRRE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: TYR31 SER33 ASN34 TYR38 TYR55 THR59 ARG60 GLU61 SER62 TYR97 TYR98 SER99 SER100 LEU102 E: SER30 SER31 TYR32 ASP33 HIS35 TRP47 ALA50 ILE51 GLY52 THR53 ALA54 ASP56 TYR58 TYR59 ASN73 TRP99 PHE100 GLY101 GLU102 LEU103 TYR104 ASP106 TYR107 S: GLU112 LYS114 LYS115 PRO116 ASP117 GLY118 SER119 LYS140 SER142 GLY143 THR144 PRO146 CYS147 ARG172 GLY173 GLN221 ALA222 THR223 GLY224 THR227 GLU229 GLU231 LEU233 ASN238 LEU239 TYR241 GLN243 GLY271 LYS272 ILE274 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
