Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5047 |
| PDBID: | 7TL0 |
| Chains: | HI_C |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.06 |
| Reference: | 10.1016/j.celrep.2022.111399 |
| Antibody | |
| Antibody: | SAN32-2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prefusion-stabilized human metapneumovirus (hMPV) fusion protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7TL0_H|Chain D, F, H|SAN32-2 Fab heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGESLRISCKSSGYTFTTYWITWVRQMPGKGLEWMGRIDPSDSYTDYSPSFKGHVTISVDKSINTAYLQWGSLKSSDTAIFYCARQASSGKIYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: I
Mutation: NULL
| >7TL0_I|Chain E, G, I|SAN32-2 Fab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCQASQGINYYLNWYQQKPGKAPKVLIYDASDLETGVPSRFSGGGSGTHFTFTISSLQTEDIGTYYCQQYDNLPFTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: Q100R/S101R/L110C/T127C/A140C/A147C/N153C/A185P/L219K/V231I/N322C/T365C/E453Q/V463C
| >7TL0_C|Chain A, B, C|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIACGVATAAAVTAGVAIAKCIRLESEVTAIKNCLKKTNECVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISKDLMTDAELARAISNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPCEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPQDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TRP33 ARG50 SER53 ASP54 TYR56 ASP58 SER97 SER98 GLY99 I: ASN30 TYR31 TYR32 ASP50 ALA51 SER52 ASP53 GLY66 SER67 TYR91 ASP92 ASN93 LEU94 C: GLU56 ASN57 THR59 CYS60 ALA61 ASP62 GLY63 LYS68 ASP72 LYS75 SER76 ARG79 ASN178 LYS179 ASN180 LYS181 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
