Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5040 |
| PDBID: | 7TJQ |
| Chains: | AB_C |
| Organism: | Homo sapiens, human metapneumovirus |
| Method: | EM |
| Resolution (Å): | 3.13 |
| Reference: | 10.1016/j.celrep.2022.111399 |
| Antibody | |
| Antibody: | SAN27-14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prefusion-stabilized human metapneumovirus (hMPV) fusion protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7TJQ_A|Chain A, D, H|SAN27-14 Fab heavy chain|Homo sapiens (9606) QVRLEQSGADVKKPGASVRVSCKASGYTFNGYYIHWVRQAPGQGLEWMGWINPYTGETNYAQTFRGRVTLTRDTSLKTLHLDLISLRSGDTAIYYCARGNCSISGCYYSWLDHWGQGTLVTVSS |
Light Chain: B
Mutation: NULL
| >7TJQ_B|Chain B, E, L|SAN27-14 Fab light chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQSINTWLAWYQQKPGKAPKLLIYEASSLERGVPSRFSGSGSGTGFTLTISSLQPDDFATYFCQQYNTYPRTFGQGTKVEIKRTVAAPSV |
Antigen
Chain: C
Mutation: Q100R/S101R/L110C/T127C/A140C/A147C/N153C/A185P/L219K/V231I/N322C/T365C/E453Q/V463C
| >7TJQ_C|Chain C, F, G|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIACGVATAAAVTAGVAIAKCIRLESEVTAIKNCLKKTNECVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISKDLMTDAELARAISNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPCEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPQDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR28 ASN30 GLY31 TYR32 TYR33 TRP50 TYR53 THR54 GLU56 ASN96 CYS97 SER98 ILE99 B: TYR94 C: LYS126 CYS127 ARG129 LEU130 GLU131 SER132 GLU133 THR135 ALA136 ASN139 CYS140 LYS143 CYS147 VAL148 SER149 THR150 LEU151 GLY152 CYS153 GLY154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
