Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 504 |
| PDBID: | 3HAE |
| Chains: | IG_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1073/pnas.0901425106 |
| Antibody | |
| Antibody: | 3HAE Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HLA class I histocompatibility antigen, A-2 alpha chain |
| Antigen mutation: | No |
| Durg Target: | P04439 |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >3HAE_I|Chain Q[auth H], R[auth I], S[auth O], T|Antibody heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSAYGMGWVRQAPGKGLEWVSSIGSSGGGTAYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAGELLPYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: G
Mutation: NULL
| >3HAE_G|Chain M[auth G], N[auth L], O[auth N], P[auth S]|Antibody light chain|Homo sapiens (9606) QSELTQPRSVSGSPGQSVTISCTGTSRDVGGYNYVSWYQQHPGKAPKLIIHDVIIRPSGVPDRFSGSKSGNTASLTISGLQAEDEAHYYCWSFAGSYYVFGTGTDVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGNTVEKTVAPT |
Antigen
Chain: D
Mutation: NULL
| >3HAE_D|Chain A, C[auth D], E[auth J], G[auth P]|HLA class I histocompatibility antigen, A-2 alpha chain|Homo sapiens (9606) GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: SER26 ARG27 GLY31 TYR32 ASN33 TYR34 ASP52 PHE93 GLY95 SER96 I: SER30 ALA31 SER53 SER54 GLY55 GLY56 GLY57 THR58 ALA59 TYR60 ASP62 LYS65 GLY66 LEU101 PRO102 TYR103 TYR104 D: GLU58 TYR59 ASP61 GLY62 GLU63 ARG65 LYS66 LYS68 ALA69 HIS70 GLN72 THR73 ARG75 VAL76 ASP77 THR80 LYS146 ALA149 ALA150 HIS151 VAL152 GLU154 GLN155 ALA158 TYR159 THR163 TRP167 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
