Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5031 |
| PDBID: | 7TGI |
| Chains: | B_A |
| Organism: | Ricinus communis, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1016/j.jbc.2022.101742 |
| Antibody | |
| Antibody: | V9E1 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ricinus communis ricin chain A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7TGI_B|Chain B|VHH antibody|Vicugna pacos (30538) QVQLVETGGLVQPGGSLRLSCAASGLTLDYYNIGWFRQAPGKEREWVSSISSSDGRKYYVNSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAADRDRLPSAITYEYNYWGQGTQVTVSSEPKTPKPQ |
Antigen
Chain: A
Mutation: NULL
| >7TGI_A|Chain A|Ricin chain A|Ricinus communis (3988) IFPKQYPIINFTTAGATVQSYTNFIRAVRGRLTTGADVRHEIPVLPNRVGLPINQRFILVELSNHAELSVTLALDVTNAYVVGYRAGNSAYFFHPDNQEDAEAITHLFTDVQNRYTFAFGGNYDRLEQLAGNLRENIELGNGPLEEAISALYYYSTGGTQLPTLARSFIICIQMISEAARFQYIEGEMRTRIRYNRRSAPDPSVITLENSWGRLSTAIQESNQGAFASPIQLQRRNGSKFSVYDVSILIPIIALMVYRCAPPPSSQF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLU43 ARG44 GLU45 TRP46 SER53 ASP54 ARG56 TYR58 TYR59 VAL60 ASN61 LYS64 ARG99 ASP100 ARG101 LEU102 PRO103 SER104 ALA105 ILE106 THR107 TYR108 GLU109 ASN111 A: ARG26 GLU41 GLN182 TYR183 GLY186 GLU187 ARG189 THR190 ARG193 TYR194 ARG196 SER203 LEU207 GLN223 LEU232 GLN233 ARG234 ARG235 ASN236 SER238 PHE240 ASP244 SER246 ILE247 ILE249 PRO250 ILE251 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
