Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5027 |
| PDBID: | 7TFN |
| Chains: | HL_A |
| Organism: | Human immunodeficiency virus 1, Macaca mulatta |
| Method: | EM |
| Resolution (Å): | 4.00 |
| Reference: | 10.1038/s41467-022-28424-3 |
| Antibody | |
| Antibody: | Ab1303 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP.664 envelope glycoprotein - gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7TFN_H|Chain D[auth H], E[auth I], H[auth P]|Anti-HIV-1 CD4bs antibody Fab Ab1303 - Heavy chain|Macaca mulatta (9544) QVQLQESGPGLVKPSETLSLTCAVSGGSISGNYWSWIRQSPGKGLEWIGHINDYSGRTDYNPSLKSRVTISTDTSTNQFFLTLSSVSAADTAVYYCARRDTDFWRGIYVFEFWGQGVLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: L
Mutation: NULL
| >7TFN_L|Chain F[auth J], G[auth L], I[auth Q]|CD4 binding site antibody Fab Ab1303 - light chain|Macaca mulatta (9544) QSVLTQSPSASASLGASVKLTCTLSSGLRSYTIAWYQRQRGQAPRFLLRLDSVGSHTKVDGIPDRFSGSSSGTERYLTISNLQSEDEADYFCQTWTTGIYIFGGGTRLSVLSQPKASPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >7TFN_A|Chain A, B, C|Envelope glycoprotein BG505 SOSIP.664 - gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETKKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER28 ILE29 SER30 GLY31 TYR33 TRP47 TYR53 SER54 ARG56 ASP58 TYR59 PRO61 SER62 LYS64 THR97 ASP98 PHE99 L: SER30 TYR31 THR32 ARG49 ASP51 HIS52 THR53 LYS54 TRP91 THR92 THR93 GLY94 ILE95 A: GLU49 THR51 LYS97 ASN99 GLU102 GLN103 HIS105 THR106 ILE109 SER110 ASP113 ILE194 THR198 ASN279 ASN280 ALA281 LYS282 ASN283 SER365 GLY366 GLY367 ASP368 GLU370 VAL371 LYS421 ASN425 GLN428 ARG429 ILE430 GLY431 THR455 ARG456 ASP457 GLY458 GLY459 ARG469 PRO470 GLY471 GLY472 ASP474 ARG476 ARG480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
