Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5 |
| PDBID: | 1AHW |
| Chains: | BA_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1006/jmbi.1997.1512 |
| Antibody | |
| Antibody: | 5G9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | TISSUE FACTOR |
| Antigen mutation: | No |
| Durg Target: | P13726 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1AHW_B|Chain B, E|IMMUNOGLOBULIN FAB 5G9 (HEAVY CHAIN)|Mus musculus (10090) EIQLQQSGAELVRPGALVKLSCKASGFNIKDYYMHWVKQRPEQGLEWIGLIDPENGNTIYDPKFQGKASITADTSSNTAYLQLSSLTSEDTAVYYCARDNSYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKI |
Light Chain: A
Mutation: NULL
| >1AHW_A|Chain A, D|IMMUNOGLOBULIN FAB 5G9 (LIGHT CHAIN)|Mus musculus (10090) DIKMTQSPSSMYASLGERVTITCKASQDIRKYLNWYQQKPWKSPKTLIYYATSLADGVPSRFSGSGSGQDYSLTISSLESDDTATYYCLQHGESPYTFGGGTKLEINRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >1AHW_C|Chain C, F|TISSUE FACTOR|Homo sapiens (9606) SGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP1 GLN27 ARG30 TYR32 TYR50 HIS91 GLY92 GLU93 SER94 PRO95 TYR96 B: PHE27 LYS30 ASP31 TYR32 TYR33 LEU50 ASP52 GLU54 ASN55 ASN57 THR58 ILE59 TYR60 GLN65 ASP99 ASN100 SER101 TYR102 C: LYS122 LYS149 ASP150 ILE152 THR154 TYR156 TYR157 GLY164 LYS165 LYS166 THR167 ALA168 LYS169 THR170 ASN171 ASP178 GLN190 VAL192 PRO194 ARG196 THR197 VAL198 ARG200 LYS201 SER202 THR203 ASP204 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
