Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 499 |
| PDBID: | 3H3B |
| Chains: | D_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.45 |
| Reference: | 10.1074/jbc.M111.235184 |
| Antibody | |
| Antibody: | chA21 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Receptor tyrosine-protein kinase erbB-2 extracellular domain |
| Antigen mutation: | No |
| Durg Target: | P04626 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >3H3B_D|Chain C, D|anti-ErbB2 antibody chA21|Mus musculus (10090) AAQPADIVLTQTPSSLPVSVGEKVTMTCKSSQTLLYSNNQKNYLAWYQQKPGQSPKLLISWAFTRKSGVPDRFTGSGSGTDFTLTIGSVKAEDLAVYYCQQYSNYPWTFGGGTRLEIKRGGGGSGGGGSGGGGSGGGGSEVQLQQSGPEVVKTGASVKISCKASGYSFTGYFINWVKKNSGKSPEWIGHISSSYATSTYNQKFKNKAAFTVDTSSSTAFMQLNSLTSEDSAVYYCVRSGNYEEYAMDYWGQGTSVTVSS |
Antigen
Chain: B
Mutation: NULL
| >3H3B_B|Chain A, B|Receptor tyrosine-protein kinase erbB-2|Homo sapiens (9606) GSTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLN-2 TYR31 ASN33 ASN34 GLN35 LYS36 TYR38 TRP56 SER98 ASN99 TYR100 TRP102 THR164 GLY165 PHE167 HIS184 SER186 SER188 TYR189 THR191 SER192 THR193 GLY234 ASN235 TYR236 GLU237 TYR239 B: PRO100 LEU101 ASN102 ASN103 THR104 THR105 VAL107 ARG135 ASN136 PRO137 TYR141 ASP143 THR144 ASP163 THR164 ASN165 ARG166 SER167 ARG168 ALA169 CYS170 HIS171 PRO172 CYS173 SER174 PRO175 GLU185 SER186 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
