Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4986 |
| PDBID: | 7T9M |
| Chains: | HL_R |
| Organism: | Mus musculus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/s41586-022-05159-1 |
| Antibody | |
| Antibody: | CS-17 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | thyrotropin receptor (TSHR) |
| Antigen mutation: | No |
| Durg Target: | P16473 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7T9M_H|Chain A[auth H]|CS-17 Heavy Chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKMSCKASGYTFTSYIIHWVKQKPGQGLEWIGYINLYNDGTNYNEKFTGKATLTSDKSSSTAYMELSSLTSEDSAVYYCAREDYYGRVADFDVWGAGTTVTVSSKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKI |
Light Chain: L
Mutation: NULL
| >7T9M_L|Chain B[auth L]|CS-17 Light Chain|Mus musculus (10090) HLVLTQSPAIMSASPGEKVTISCSASSSVSYMCWFQQKPGSSPKPWIYRTSNLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCQQYHSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: R
Mutation: NULL
| >7T9M_R|Chain C[auth R]|Thyrotropin receptor|Homo sapiens (9606) DYKDDDDGTMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHEMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ILE33 TYR50 ASN52 TYR54 ASN55 ASP56 GLY57 THR58 ASN59 TYR60 GLU62 THR65 GLU99 ASP100 TYR101 TYR102 GLY103 ASP107 L: HIS1 SER30 TYR31 ARG49 TYR90 HIS91 SER92 TYR93 PRO94 R: TYR195 ASN198 VAL215 ASP217 LYS218 ASP219 GLY223 VAL224 TYR225 ALA240 LEU241 PRO242 SER243 LYS244 GLY245 GLU247 HIS248 LYS262 PRO264 LEU267 SER268 LEU270 ASN483 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
