Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4985 |
| PDBID: | 7T87 |
| Chains: | HL_B |
| Organism: | Staphylococcaceae, Mus musculus, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.chom.2023.04.004 |
| Antibody | |
| Antibody: | 214 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Staphylococcaceae leukocidin A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7T87_H|Chain D[auth H]|Antibody Fab B214 Heavy Chain|Mus musculus (10090) EVQLQQSGAELVKPGASVKISCKASGYAFSSSWMNWLKQRPGKGLEWIGRIYPGDGDTNYNGKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYFCARYGYDYDGEYYYAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >7T87_L|Chain C[auth L]|Antibody Fab B214 Light Chain|Mus musculus (10090) DIVMTQSPTTMAASPGERITITCSAHSNLISNYLHWYQQKPGFSPKLLIYRTSNLASGVPARFSGSGSGTSYSLTIGTMEAEDVATYFCQQGSSIPFTFGSGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: B
Mutation: NULL
| >7T87_B|Chain B|Leukocidin A|Staphylococcaceae (90964) NSAHHHHHHGSHKDSQDQNKKEHVDKSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYDKNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNKISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHWSVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLTYLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKTVKVVDKYSDDNKPYKEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN32 ARG51 SER68 H: TYR103 ASP104 B: GLU56 PHE93 ASN224 GLU226 LYS232 TYR233 ARG240 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
