Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4981 |
| PDBID: | 7T4S |
| Chains: | HI_D |
| Organism: | Human betaherpesvirus 5, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1126/sciadv.abm2536 |
| Antibody | |
| Antibody: | 8I21 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope protein UL130 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7T4S_H|Chain G[auth H]|Fab 8I21 heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYALVELVESGGGVVQPGRSLRLSCAASGFTFSSDGMHWVRQSPGRGLEWVAFISSDGSTPYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAMYFCAKDWALFRWLRTFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: I
Mutation: NULL
| >7T4S_I|Chain J[auth I]|Fab 8I21 light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAETVMTQSPATLSVSPGGRATLSCRASQSVGINLAWYQQKPGQAPRLLIYGASTRASGFPARFSGSGSGTEFTLTITSLQSEDFAVYYCQQYNDWPPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >7T4S_D|Chain D|Envelope glycoprotein UL130|Human betaherpesvirus 5 (10359) MLRLLLRHHFHCLLLCAVWATPCLASPWFTLTANQNPSPPWSKLTYPKPHDAATFYCPFLYPSPPRSPSQFSGFQRVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQTILQRMPRTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWAHVFRDYSVSFQVRLTFTEANNQTYTFCTHPNLIVGSENLYFQGSAWSHPQFEKGGGSGGGSGGGSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PHE50 SER52 ASP54 SER56 THR57 TYR59 PHE103 ARG104 TRP105 LEU106 ARG107 I: ARG18 THR20 GLN27 SER28 GLY30 ILE31 ASN32 SER52 THR53 ARG54 ALA60 ARG61 SER63 GLY64 SER65 GLY66 SER67 THR72 THR74 THR76 SER77 ASN92 ASP93 TRP94 PRO95 D: TYR46 PRO47 LYS48 PRO49 ALA52 SER72 GLY73 PHE74 GLN75 ARG76 SER78 LEU90 TYR92 ASN93 GLY96 THR98 LEU114 SER115 ASN118 GLN119 THR120 GLN123 ARG124 VAL137 GLN138 ILE139 SER140 VAL141 GLU142 ASP143 ILE146 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
