Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4976 |
| PDBID: | 7T4R |
| Chains: | GH_B |
| Organism: | Homo sapiens, Human betaherpesvirus 5 |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1126/sciadv.abm2536 |
| Antibody | |
| Antibody: | 13H11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope glycoprotein H |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7T4R_G|Chain G, P|Fab 13H11 heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAQVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYIHWVRQAPGQGLEWMGIIHPSSGGTSYAQKFQGRVTMTRDTSTSTVSMDLSSLRSEDTAVYYCGRAFRILGLSDVFVNDWGQGTVVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: H
Mutation: NULL
| >7T4R_H|Chain H, Q|Fab 13H11 light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYADIQMTQSPSSLSASVGDRVTITCRASQGINNYLAWYQQKPGKVPKLLIYAASTLQSGVPSRFSGSGSGTAFTLTILSLQPEDVATYYCQKYNSAPFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7T4R_B|Chain B, K|Envelope glycoprotein H|Human betaherpesvirus 5 (10359) MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCTYNSSLRNSTVVRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQRLNTYALVSKDLASYRSFSQQLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTESHTTSGLHRPHFNQTCILFDGHDLLFSTVTPCLHQGFYLIDELRYVKITLTEDFFVVTVSIDDDTPMLLIFGHLPRVLFKAPYQRDNFILRQTEKHELLVLVKKDQLNRHSYLKDPDFLDAALDFNYLDLSALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFAAARQEEAGAQVSVPRALDRQAALLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLVRLVYILSKQNQQHLIPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVHTTERREIFIVETGLCSLAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFPDATVPATVPAALSILSTMQPSTLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGISYPVSTTVVGQSLIITQTDSQTKCELTRNMHTTHSITVALNISLENCAFCQSALLEYDDTQGVINIMYMHDSDDVLFALDPYNEVVVSSPRTHYLMLLKNGTVLEVTDVVVDATDGTKLGPEQKLISEEDLNSAVDGSGLNDIFEAQKIEWHENLYFQGHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: ASN31 TYR33 ILE50 ILE51 HIS52 SER54 SER55 GLY57 THR58 SER59 ILE102 LEU103 GLY104 LEU105 SER106 VAL108 H: ASP1 ILE2 GLN3 THR5 ARG24 ALA25 SER26 GLN27 GLY28 ILE29 ASN30 ASN31 TYR32 SER65 GLY66 SER67 GLY68 THR69 ALA70 THR72 ASN92 SER93 ALA94 PRO95 PHE96 B: LEU218 ILE219 ASP220 GLU221 LEU222 ARG223 ILE240 ASP241 ASP242 ASP243 ASN287 ARG288 GLY328 ARG329 CYS330 GLN331 MET332 GLN386 THR387 PRO388 ARG528 HIS530 SER531 LEU532 GLU533 PRO549 LEU552 SER553 SER556 THR557 GLN559 SER561 PRO573 LEU574 GLY575 GLU576 SER577 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
