Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4975 |
| PDBID: | 7T4Q |
| Chains: | JK_A |
| Organism: | Human betaherpesvirus 5, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.90 |
| Reference: | 10.1126/sciadv.abm2536 |
| Antibody | |
| Antibody: | 13H11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope glycoprotein H |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >7T4Q_J|Chain J|Fab 13H11 heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAQVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYIHWVRQAPGQGLEWMGIIHPSSGGTSYAQKFQGRVTMTRDTSTSTVSMDLSSLRSEDTAVYYCGRAFRILGLSDVFVNDWGQGTVVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: K
Mutation: NULL
| >7T4Q_K|Chain K|Fab 13H11 light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYADIQMTQSPSSLSASVGDRVTITCRASQGINNYLAWYQQKPGKVPKLLIYAASTLQSGVPSRFSGSGSGTAFTLTILSLQPEDVATYYCQKYNSAPFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7T4Q_A|Chain A|Envelope glycoprotein H|Human betaherpesvirus 5 (10359) MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCTYNSSLRNSTVVRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQRLNTYALVSKDLASYRSFSQQLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTESHTTSGLHRPHFNQTCILFDGHDLLFSTVTPCLHQGFYLIDELRYVKITLTEDFFVVTVSIDDDTPMLLIFGHLPRVLFKAPYQRDNFILRQTEKHELLVLVKKDQLNRHSYLKDPDFLDAALDFNYLDLSALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFAAARQEEAGAQVSVPRALDRQAALLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLVRLVYILSKQNQQHLIPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVHTTERREIFIVETGLCSLAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFPDATVPATVPAALSILSTMQPSTLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGISYPVSTTVVGQSLIITQTDSQTKCELTRNMHTTHSITVALNISLENCAFCQSALLEYDDTQGVINIMYMHDSDDVLFALDPYNEVVVSSPRTHYLMLLKNGTVLEVTDVVVDATDGTKLGPEQKLISEEDLNSAVDGSGLNDIFEAQKIEWHENLYFQGHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: ASN54 TYR56 ILE73 HIS75 SER77 SER78 GLY80 THR81 SER82 TYR83 GLN85 GLN88 ILE125 LEU126 GLY127 LEU128 SER129 VAL131 K: ASP24 ILE25 GLN26 THR28 SER30 ARG47 ALA48 SER49 GLN50 GLY51 ILE52 ASN53 TYR55 SER88 GLY89 SER90 GLY91 THR92 ALA93 THR95 TYR114 ASN115 SER116 ALA117 PRO118 PHE119 A: LEU218 ILE219 ASP220 GLU221 LEU222 ARG223 ILE240 ASP241 ASP242 ASP243 ASN287 ARG288 GLY328 ARG329 CYS330 GLN331 MET332 GLN386 THR387 PRO388 ARG528 HIS530 SER531 LEU532 GLU533 PRO549 LEU552 SER553 SER556 THR557 GLN559 SER561 LEU574 GLY575 GLU576 SER577 ASN593 GLN594 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
