Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4974 |
| PDBID: | 7T4Q |
| Chains: | HI_D |
| Organism: | Human betaherpesvirus 5, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.90 |
| Reference: | 10.1126/sciadv.abm2536 |
| Antibody | |
| Antibody: | 7I13 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope protein UL130 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7T4Q_H|Chain H|Fab 7I13 light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEIVLTQSPGTLSLSPGERATLSCRASQSVSSDFLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYAASPPFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Light Chain: I
Mutation: NULL
| >7T4Q_I|Chain I|Fab 7I13 heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAQVQLVESGGGVVQPGRSLRLSCAASGFTFSNYGMHWVRQGPGKGLEWVAVISYDASSKYYTDSVQGRFTISRDNSKNTLFLQMNSLRGEDTAVYYCAKALRYLDWFLSDPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Antigen
Chain: D
Mutation: NULL
| >7T4Q_D|Chain D|Envelope glycoprotein UL130|Human betaherpesvirus 5 (10359) MLRLLLRHHFHCLLLCAVWATPCLASPWFTLTANQNPSPPWSKLTYPKPHDAATFYCPFLYPSPPRSPSQFSGFQRVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQTILQRMPRTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWAHVFRDYSVSFQVRLTFTEANNQTYTFCTHPNLIVGSENLYFQGSAWSHPQFEKGGGSGGGSGGGSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU24 ILE25 GLN50 SER51 VAL52 SER53 PHE56 GLN114 TYR115 ALA116 ALA117 SER118 I: TRP70 VAL73 TYR82 TYR83 ASP85 GLN88 ASP127 TRP128 LEU130 SER131 D: ARG160 VAL162 ASN164 THR167 ARG168 TYR169 MET171 GLU198 ALA199 ASN200 ASN201 GLN202 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
