Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4973 |
| PDBID: | 7T4Q |
| Chains: | FG_E |
| Organism: | Human betaherpesvirus 5, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.90 |
| Reference: | 10.1126/sciadv.abm2536 |
| Antibody | |
| Antibody: | 2C12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope protein UL131A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7T4Q_F|Chain F|Fab 2C12 heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAQITLRESGPTLVKPTQTLTLTCTFSGFSLNTNGVGVGWIRQPPGKALEWLALIYWNGNEGYSPSLKSRLTITKDTSKNQVVLTMTNMDPVDTATYYCVHWPQGLTTVTRLAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: G
Mutation: NULL
| >7T4Q_G|Chain G|Fab 2C12 light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAQSALTQPRSVSGSPGQSVTISCTGTTSDVGRYNFVSWYQQHPGKAPKLLMYDVSQRPSGVPSRFSGSKSGNTASLTISGLQAEDEAVFYCCSYAGGNFFSYVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: E
Mutation: NULL
| >7T4Q_E|Chain E|Envelope protein UL131A|Human betaherpesvirus 5 (10359) MRLCRVWLSVCLCAVVLGQCQRETAEKNDYYRVPHYWDACSRALPDQTRYKYVEQLVDLTLNYHYDASHGLDNFDVLKRINVTEVSLLISDFRRQNRRGGTNKRTTFNAAGSLAPHARSLEFSVRLFAN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: TYR77 TRP78 ASN79 ASN81 GLU82 THR129 VAL130 THR131 LEU133 G: ARG54 TYR55 PHE57 TYR116 GLY119 ASN120 PHE121 PHE122 E: THR24 ALA25 LYS27 ASN28 ASP29 TYR30 TYR31 VAL33 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
