Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 497 |
| PDBID: | 3GRW |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1172/JCI38017 |
| Antibody | |
| Antibody: | R3Mab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Fibroblast growth factor receptor 3(FGFR3) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3GRW_H|Chain C[auth H]|Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFTSTGISWVRQAPGKGLEWVGRIYPTNGSTNYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARTYGIYDLYVDYTEYVMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >3GRW_L|Chain B[auth L]|Fab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >3GRW_A|Chain A|Fibroblast growth factor receptor 3|Homo sapiens (9606) ADPDTGVDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGREFRGEHRIGGIKLRHQQWSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSDAQPHIQWLKHVEVNGSKVGPDGTPYVTVLKSWISESVEADVRLRLANVSERDGGEYLCRATNFIGVAEKAFWLSVHGPRAAEEELVEADEAGSVHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER30 THR31 TYR49 SER50 SER52 PHE53 LEU54 TYR55 SER56 H: GLU1 GLN3 ALA24 SER25 GLY26 PHE27 THR28 THR30 SER31 THR32 PRO52 THR73 SER74 LYS75 ASN76 ARG94 TYR96 GLY97 ILE98 TYR99 A: ARG155 ARG158 MET159 LYS161 LYS162 LEU163 LEU164 ALA165 VAL166 PRO167 ALA168 ALA169 ASN170 THR171 VAL172 ARG173 PHE174 ARG175 PRO177 LYS205 ARG207 GLN210 VAL214 GLU216 TYR241 LEU246 GLU247 ARG248 TYR278 SER279 ASP280 ALA281 GLN282 PRO283 GLU315 SER316 VAL317 GLU318 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
