Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4965 |
| PDBID: | 7T3D |
| Chains: | JM_E |
| Organism: | Influenza A virus (A/California/04/2009(H1N1)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.38 |
| Reference: | 10.1038/s41586-021-04356-8 |
| Antibody | |
| Antibody: | 045-09 2B05 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin HA1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >7T3D_J|Chain D[auth C], J, P[auth K]|2B05 mAb heavy chain|Homo sapiens (9606) VQLLESGGGLVQPGGSLSLSCAASGFTFSSFAMSWVRQAPVKGLEWVSMISAGGGNTYYADSVKGRFTISRDNSKSTLYLQMSSLTAEDTAVYYCAKSDSSGFQYGRREFWGQGTLVTVS |
Light Chain: M
Mutation: NULL
| >7T3D_M|Chain C[auth D], I[auth M], O[auth N]|2B05 mAb light chain|Homo sapiens (9606) DIQMTQSPSSLSAFVGDRVTIACQASQDIRIHLNWYQQKPGKAPKLLIYDASNLEAGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQHYHNLPRTFGGGTKVEIK |
Antigen
Chain: E
Mutation: NULL
| >7T3D_E|Chain A, G[auth E], M[auth F]|Hemagglutinin HA1 chain|Influenza A virus (A/California/04/2009(H1N1)) (641501) ADPGDTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: ARG30 ILE31 HIS32 TYR49 ASN53 LEU54 GLU55 ALA56 TYR91 HIS92 J: THR28 SER30 SER31 PHE32 ALA52 GLY53 GLY55 ASN56 ASP96 SER97 SER98 GLY99 ARG100 E: GLU77 LEU79 SER80 THR81 PHE118 GLU119 ARG120 PHE121 GLU122 ILE123 SER125 SER126 LYS166 TYR168 ILE169 ASN170 ASP171 LYS172 LYS174 VAL176 TYR256 ALA259 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
