Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4963 |
| PDBID: | 7T2P |
| Chains: | HL_A |
| Organism: | Simian immunodeficiency virus, Macaca mulatta |
| Method: | EM |
| Resolution (Å): | 3.47 |
| Reference: | 10.1038/s41467-022-32783-2 |
| Antibody | |
| Antibody: | K11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Simian immunodeficiency viruses SIVmac239.K180S SOSIP envelope glycoprotein - gp120 |
| Antigen mutation: | Yes |
| Durg Target: | P05884 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7T2P_H|Chain C[auth H]|K11 Fab heavy chain|Macaca mulatta (9544) DWTWRILFLVAAATGAHSQVQLQESGPGVVRPSQTLSLTCAVSGDTVSSCCFFWTWIRQPPGKGLEWIGNISYDNDNTNYNPSLKTRISISKDMSKNQFSLKLNSLTATDTAIYYCARESPSRGNFCYAYLYGNCPLHFDLWGQGVLVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIKTCGGGSKPPTCPPCTSPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPDVKFNWYVNGAEVHHAQTKPRETQYNSTYRVVSVLTVTHQDWLNGKEYTCKVSNKALPAPIQKTISKDKGQPREPQVYTLPPSREELTKNQVSLTCLVKGFYPSDIVVEWESSGQPENTYKTTPPVLDSDGSYFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
Light Chain: L
Mutation: NULL
| >7T2P_L|Chain D[auth L]|K11 Fab light chain|Macaca mulatta (9544) ETDTLLLWVLLLWVPGSTGDILLTQSPSSLSGSVGDRVTITCRASQGINSYLNWYQQKPGKAPKLLIYFANRLQSGVPSRFSGSGSGTEFTLTISSLQSEDGATYYCQQYDTFPTFGPGTKLDIKRTVAAPSVFIFPPSEDQVKSGTVSVVCLLNNFYPREASVKWKVDGALKTGNSQESVTEQDSKDNTYSLSSTLTLSSTEYQSHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: K180S
| >7T2P_A|Chain A|Envelope glycoprotein gp120|Simian immunodeficiency virus (11723) MGCLGNQLLIAILLLSVYGIYCTLYVTVFYGVPAWRNATIPLFCATKNRDTWGTTQCLPDNGDYSEMALNVTESFDAWNNTVTEQAIEDVWQLFETSIKPCVKLSPLCITMRCNKSETDRWGLTKSITTTASTTSTTASAKVDMVNETSSCIAQDNCTGLEQEQMISCKFNMTGLKRDKSKEYNETWYSADLVCEQGNNTGNESRCYMNHCNTSVIQESCDKHYWDAIRFRYCAPPGYALLRCNDTNYSGFMPKCSKVVVSSCTRMMETQTSTWFGFNGTRAENRTYIYWHGRDNRTIISLNKYYNLTMKCRRPGNKTVLPVTIMSGLVFHSQPINDRPKQAWCWFGGKWKDAIKEVKQTIVKHPRYTGTNNTDKINLTAPGGGDPEVTFMWTNCRGEFLYCKMNWFLNWVEDRNTANQKPKEQHKRNYVPCHIRQIINTWHKVGKNVYLPPREGDLTCNSTVTSLIANIDWIDGNQTNITMSAEVAELYRLELGDYKLVEITPIGLAPTSCKRYTTGGTSRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: CYS32 ASP53 ARG99 L: ASN30 TYR32 ASP92 A: ASP245 ASN247 SER249 PHE251 MET252 PRO253 LYS254 SER256 GLY292 VAL362 LYS363 HIS364 PRO365 ARG366 TYR367 THR368 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
