Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4952 |
| PDBID: | 7SWP |
| Chains: | HL_A |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.80 |
| Reference: | 10.1126/sciimmunol.abo3425 |
| Antibody | |
| Antibody: | G32Q4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike hexapro (6P) glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7SWP_H|Chain B[auth H]|G32Q4 Fab heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFSRYAMQWVRQAPGKGLEWVAVISYDGNNRYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGPPGYYDSSGYYQTPEYFQHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >7SWP_L|Chain C[auth L]|G32Q4 Fab light chain|Homo sapiens (9606) QSVLTQPPSVSGAPGQRVTISCTGSSSNIGVGYDVHWYQQFPGTVPKLLIYGNSNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDSSLSGVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >7SWP_A|Chain A|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) NLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLY26 PHE27 THR28 ARG31 TYR32 TYR53 ARG98 PRO100 PRO101 GLY102 TYR103 TYR104 ASP105 SER106 SER107 GLY108 GLU114 TYR115 GLN117 L: ASP34 HIS36 TYR51 GLY52 ASN53 SER54 ASN55 ARG56 PRO57 SER58 GLY66 SER67 LYS68 A: TYR369 PHE377 LYS378 CYS379 TYR380 GLY381 VAL382 SER383 PRO384 THR385 LYS386 ARG403 ASP405 GLU406 ARG408 GLN409 ALA411 PRO412 GLY413 GLN414 THR415 GLY416 LYS417 ASP420 ASP427 ASP428 PHE429 THR430 VAL503 GLY504 TYR505 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
