Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 495 |
| PDBID: | 3GI8 |
| Chains: | HL_C |
| Organism: | Methanocaldococcus jannaschii, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.59 |
| Reference: | 10.1126/science.1176088 |
| Antibody | |
| Antibody: | 7F11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Amino acid, polyamine, and organocation (APC) transporters |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3GI8_H|Chain C[auth H]|7F11 Anti-ApcT Monoclonal Fab Heavy Chain|Mus musculus (10090) QVQLLQPGAELVKPGASVKLSCKASGYTFTSYWMYWVKQRPGQGLEWIGEIDPSDSYTNYNQNFKGKATLTVDKSSSTAFMQLSSLTSQDSAVYFCARSPHYYGTTYNYPMDYWGQGTSVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >3GI8_L|Chain B[auth L]|7F11 Anti-ApcT Monoclonal Fab Light Chain|Mus musculus (10090) DIVMTQSPSSLAMSVGQKVTLSCKSSQSLLNTSNQKNYLAWYQQKPGQSPKLLVYFASTRESGVPDRFIGSGSGTDFTLTISSVQAEDLSDFFCQQHYSTPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: K158A
| >3GI8_C|Chain A[auth C]|Uncharacterized protein MJ0609|Methanocaldococcus jannaschii (2190) MELKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDNIITGALSILLWMSYVISIALFAKGFAGYFLPLINAPINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVALLILGLFIFAGLITIHPSYVIPDLAPSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIFISILIVMFVYVGVAISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFERKVWFKSTEGLYITSALGVLFALLFNMEGVASITSAVFMVIYLFVILSHYILIDEVGGRKEIVIFSFIVVLGVFLLLLYYQWITNRFVFYGIIATFIGVLIFEIIYRKVTKRTFSNNMYVKSLESSGLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: LEU52 TYR55 GLU61 SER62 H: GLY26 TYR27 THR28 THR30 SER31 TYR32 ASP52 SER54 ASP55 LYS74 SER75 ARG98 PRO100 HIS101 TYR102 TYR103 GLY104 THR105 TYR107 TYR109 PRO110 ASP112 TYR113 C: MET1 GLU2 LEU3 LYS4 ASN5 LYS6 LYS145 LYS304 ASP305 GLU307 LEU308 PRO309 GLU310 PHE311 PHE312 GLU313 VAL316 PHE318 LYS319 SER320 THR426 ASN430 LYS434 SER435 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
